Journal: iScience

Article Title: Classifying Drosophila olfactory projection neuron boutons by quantitative analysis of electron microscopic reconstruction

doi: 10.1016/j.isci.2022.104180

Figure Lengend Snippet: Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron Microscopy (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.

Article Snippet: Serial EM images were then acquired by focused ion beam scanning electron microscopy (FIB-SEM, Helios Nanolab 600i), which is controlled by Auto Slice & View software.

Techniques: Electron Microscopy

Journal: Materials (Basel, Switzerland)

Article Title: Polyurethane/ n -Octadecane Phase-Change Microcapsules via Emulsion Interfacial Polymerization: The Effect of Paraffin Loading on Capsule Shell Formation and Latent Heat Storage Properties.

doi: 10.3390/ma16196460

Figure Lengend Snippet: Figure 1. Confocal microscopy images of the PU capsules at different reaction stages: (a) as-prepared PVA emulsion (the red arrow shows the liquid content released due to disruption of PVA layer, the black arrow shows an intact emulsion droplet); (b) at the beginning of the shell polymerization after addition of PEG and TMA; (c) PU capsules after completion of the reaction.

Article Snippet: The morphology and shell thickness of the prepared capsules were studied with scanning electron microscopy (SEM) employing an SEM-FIB JIB-4501 (JEOL, Akishima/Tokyo, Japan) microscope.

Techniques: Confocal Microscopy, Capsules, Emulsion, Disruption